QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26649APC
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Neutralization, Flow Cytometry, Immunoprecipitation
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6A8.6F10.1A6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells.The antibody reacts with the Qa-1b Class Ib molecule.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]
Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin] [NBP2-26649APC] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review/
Applications for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-26667. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Reviewed Applications
Read 1 review rated 4 using NBP2-26649APC in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: QA1b
Alternate Names
H2-Q1, H2-T23
Gene Symbol
QA1b
Additional QA1b Products
Product Documents for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]
Product Specific Notices for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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