QA1b Antibody (6A8.6F10.1A6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26649
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, Block/Neutralize, Flow Cytometry
Cited:
Block/Neutralize, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6A8.6F10.1A6
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the Qa-1b Class Ib molecule.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for QA1b Antibody (6A8.6F10.1A6) - BSA Free
Western Blot: QA1b Antibody (6A8.6F10.1A6)BSA Free [NBP2-26649]
Western Blot: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in mouse thymus tissue lysate using NBP2-26649 at 2 ug/mL.Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) - BSA Free [NBP2-26649]
Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. CNS cells of EAE mice stained for viable cells using APC conjugated QA1B antibody (NBP2-26649APC). Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review.Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) - BSA Free [NBP2-26649]
Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells.Applications for QA1b Antibody (6A8.6F10.1A6) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Western Blot
1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: QA1b
Additional QA1b Products
Product Documents for QA1b Antibody (6A8.6F10.1A6) - BSA Free
Product Specific Notices for QA1b Antibody (6A8.6F10.1A6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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