Phospho-Tyrosine Alexa Fluor™ Plus 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1676AFP405

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FAB1676AFP405-100UG
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Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 179003

View all Phospho-Tyrosine Antibodies »

Product Specifications

Immunogen

KLH-coupled phospho-tyrosine synthetic peptide

Specificity

Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence.No cross‑reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Phospho-Tyrosine

Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal cell cycle progression and oncogenic transformation. The role  of tyrosine phosphorylation is a matter of active and ongoing research. Specific antibodies that  recognize phosphorylated tyrosine residues are valuable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they are involved.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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