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Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 179003

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

KLH-coupled phospho-tyrosine synthetic peptide

Modification

p Tyr

Specificity

Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence.No cross‑reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Phospho-Tyrosine [p Tyr] Antibody (179003) [mFluor Violet 500 SE]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Phospho-Tyrosine

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine [p Tyr] Antibody (179003) [mFluor Violet 500 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phospho-Tyrosine [p Tyr] Antibody (179003) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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