Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # APM1676
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Multi-Species
Applications
ELISA, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Monoclonal Mouse IgG1 Clone # 179003
Product Specifications
Specificity
Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody
Phospho-PDGF R beta ELISA Using APM1676 as Detection Antibody.
Detection of Phospho-Tyrosine by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.Applications for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody
Application
Recommended Usage
ELISA
Western Blot
1:1000 dilution
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers
and preservatives.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Phospho-Tyrosine
Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody
Product Specific Notices for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody
For research use only
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