MGC20410 Antibody (PCRP-BATF2-2B9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20601
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PCRP-BATF2-2B9
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa5-140) of human MGC20410 (exact sequence is proprietary)
Localization
Nucleus.
Specificity
This antibody recognizes a transcription factor involved in differentiation of CD8+ thymic dendritic cells MGC20410 has been implicated in breast cancer, malignant glioma and metastatis melanoma progression
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Positive Controls: Human spleen, colon or pancreas.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for MGC20410 Antibody (PCRP-BATF2-2B9)
Flow cytometric analysis of PFA-fixed HeLa cells. BATF2 MGC20410 antibody (PCRP-BATF2-2B9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
MGC20410 Antibody (PCRP-BATF2-2B9)
Analysis of Protein Array containing more than 19,000 full-length human proteins using MGC20410 Mouse Monoclonal Antibody (PCRP-BATF2-2B9).MGC20410 Antibody (PCRP-BATF2-2B9)
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with MGC20410 antibody (PCRP-BATF2-2B9). Inset: PBS instead of primary antibody; secondary only negative control.Applications for MGC20410 Antibody (PCRP-BATF2-2B9)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug of protein or 1ml of lysate
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MGC20410
Alternate Names
basic leucine zipper transcription factor, ATF-like 2, basic leucine zipper transcriptional factor ATF-like 2, B-ATF-2, MGC20410, SARI, suppressor of AP-1 regulated by IFN
Gene Symbol
BATF2
Additional MGC20410 Products
Product Documents for MGC20410 Antibody (PCRP-BATF2-2B9)
Product Specific Notices for MGC20410 Antibody (PCRP-BATF2-2B9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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