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MGC20410 Antibody (PCRP-BATF2-2B9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20601

Novus Biologicals, part of Bio-Techne
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NBP3-20601-20ug
NBP3-20601-100ug

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PCRP-BATF2-2B9

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa5-140) of human MGC20410 (exact sequence is proprietary)

Localization

Nucleus.

Specificity

This antibody recognizes a transcription factor involved in differentiation of CD8+ thymic dendritic cells MGC20410 has been implicated in breast cancer, malignant glioma and metastatis melanoma progression

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Positive Controls: Human spleen, colon or pancreas.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for MGC20410 Antibody (PCRP-BATF2-2B9)

Flow cytometric analysis of PFA-fixed HeLa cells. BATF2 MGC20410 antibody (PCRP-BATF2-2B9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
MGC20410 Antibody (PCRP-BATF2-2B9)

MGC20410 Antibody (PCRP-BATF2-2B9)

Analysis of Protein Array containing more than 19,000 full-length human proteins using MGC20410 Mouse Monoclonal Antibody (PCRP-BATF2-2B9).
MGC20410 Antibody (PCRP-BATF2-2B9)

MGC20410 Antibody (PCRP-BATF2-2B9)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with MGC20410 antibody (PCRP-BATF2-2B9). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for MGC20410 Antibody (PCRP-BATF2-2B9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug of protein or 1ml of lysate
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MGC20410

BATF2 (Basic leucine zipper transcriptional factor ATF-like 2) is a 274 amino acid member of the AP-1 transcription factor family. It controls the differentiation of lineage-specific cells in the immune system. It participates in differentiating CD8+ thymic conventional dendritic cells and acts by forming a heterodimer with JUN family proteins. They then recognize and bind the DNA sequence 5'-TGA(CG)TCA-3'. It acts as a tumor suppressor by interfering with AP-1 regulated activation of CYR61/CCN1 transcription and its downstream signaling. CYR61/CCN1 signaling induces anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma.

Alternate Names

basic leucine zipper transcription factor, ATF-like 2, basic leucine zipper transcriptional factor ATF-like 2, B-ATF-2, MGC20410, SARI, suppressor of AP-1 regulated by IFN

Gene Symbol

BATF2

Additional MGC20410 Products

Product Documents for MGC20410 Antibody (PCRP-BATF2-2B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MGC20410 Antibody (PCRP-BATF2-2B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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