Human Claudin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4618
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 421203
Product Specifications
Immunogen
CHO Chinese hamster ovary cell line transfected with human Claudin-1
Met1-Val211
Accession # O95832
Met1-Val211
Accession # O95832
Specificity
Stains human Claudin-1 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Claudin-1 Antibody
Detection of Claudin-1 in PC-3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).
Immunofluorescent Staining of iPSC-derived Pancreatic Duct-like Organoids
(A) iPSC-derived pancreatic duct-like organoids were fixed and stained with a Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075; green) and Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red) or with a (B) Mouse Anti-Human KRT19 Monoclonal Antibody (Catalog # MAB35061; green) and Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red), followed by secondary antibody staining with the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009) and NorthernLights 557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid. Images were taken using a Leica THUNDER Imaging System (scale bar: 20 mm).Applications for Human Claudin-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: PC-3 human prostate cancer cell line
Sample: PC-3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 4 using MAB4618 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Claudin-1
Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.
Alternate Names
Claudin1, CLD1, CLDN1, SEMP1
Gene Symbol
CLDN1
UniProt
Additional Claudin-1 Products
Product Documents for Human Claudin-1 Antibody
Product Specific Notices for Human Claudin-1 Antibody
For research use only
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