Dysferlin Antibody (CL10888) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07994

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10888

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dysferlin Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O75923, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Mouse, Rat (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Dysferlin Antibody (CL10888) - BSA Free (NBP3-07994) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Dysferlin Antibody (CL10888) - BSA Free

Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994]

Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994]

Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human spleen and skin tissues using NBP3-07994 antibody. Corresponding Dysferlin RNA-seq data are presented for the same tissues.
Western Blot: Dysferlin Antibody (CL10888) [NBP3-07994]

Western Blot: Dysferlin Antibody (CL10888) [NBP3-07994]

Western Blot: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994]

Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994]

Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human spleen shows moderate positivity in cells in red pulp.
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Applications for Dysferlin Antibody (CL10888) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dysferlin

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Alternate Names

dysferlin, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), dystrophy-associated fer-1-like 1, FER1L1Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FLJ00175, FLJ90168, LGMD2B

Gene Symbol

DYSF

Additional Dysferlin Products

Product Documents for Dysferlin Antibody (CL10888) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dysferlin Antibody (CL10888) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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