Detects Carboxymethyl Lysine adducts on proteins in Western blots. In Western blots, no cross-reactivity with carboxyethyl lysine adducts is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Carboxymethyl Lysine Antibody

Detection of CML-adducted Protein by Western Blot.

Western blot shows 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers. PVDF membrane was probed with 1 µg/mL Carboxymethyl Lysine Monoclonal Antibody (Catalog # MAB3247) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Greater band intensity for the IgG heavy chain (IgG hc) suggests increased glycation in diabetic sera. For additional reference, carboxymethyl lysine-adducted (CML) Leptin and carboxyethyl lysine-adducted (CEL) Leptin were included. A specific band for CML-adducted Leptin was detected (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.

Applications for Carboxymethyl Lysine Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers, and carboxymethyl lysine-adducted Leptin.

Reviewed Applications

Read 1 review rated 5 using MAB3247 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxymethyl Lysine

N epsilon-carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.

Alternate Names

CML

Additional Carboxymethyl Lysine Products

Product Documents for Carboxymethyl Lysine Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Carboxymethyl Lysine Antibody

For research use only

Citations
Reviews

Carboxymethyl Lysine Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3247

Key Product Details

Species Reactivity

Validated:

Cited:

Applications