Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologics, and valued partners such as Cell Signaling Technology, Bethyl Laboratories, and OriGene are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated for Simple Western by supplier and backed by any applicable supplier guarantees
Simple Western Validated Antibodies
Filter Antibodies: (7660)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| α-Actinin-4 | Primary |
GeneTex GTX101669 |
alpha (α) Actinin 4; RRID: AB_10617403 |
Citations
(1) Datasheet |
Rabbit | UC-sEVs and SEC-sEVs | Size | 32 µg/mL | ~100 kDa |
| α-Sarcomeric actin | Primary |
MilliporeSigma A2172 |
Purified rabbit striated muscle actin used as immunogen; RRID: AB_476695 |
Citations
(1) Datasheet |
Mouse | Protein lysates | Size | 1:10,000 | |
| α-SMA | Primary |
Dako M0851 |
alpha (α) SMA; RRID: AB_2223500 |
Citations
(1) Datasheet |
Mouse | Rabbit corneal fibroblasts treated with TGF-β | Size | 1:100 | 48 |
| α-SMA | Primary |
Abcam ab21027 |
alpha (α) smooth muscle Actin; RRID: AB_1951138 |
Citations
(1) Datasheet |
Goat | Lung fibroblasts | 1:50 | ||
| α-SMA | Primary |
Invitrogen Antibodies 14-9760-82 |
alpha (α)-Smooth Muscle Actin; RRID: AB_2572996 |
Citations
(1) Datasheet |
Mouse | Fibroblasts | 1:100 | ||
| α-Synuclein | Primary |
Cell Signaling Technology 2642 |
alpha (α)-Synuclein ; RRID: AB_2192679 |
Citations
(1) Datasheet |
Rabbit | Size | 1:25 | 21 | |
| α-Tubulin | Primary |
Invitrogen Antibodies PA5-16891 |
alpha (α) Tubulin; RRID: AB_10984790 |
Citations
(1) Datasheet |
Rabbit | 1:1 | |||
| α-Tubulin | Primary |
LI-COR 926-42213 |
RRID: AB_2637092 |
Citations
(1) Datasheet |
Mouse | MCF-7,SK-BR-3,T-47D,U2OS | Size | 1:100 | |
| α-Tubulin | Primary |
Abcam ab52866 |
alpha (α) Tubulin [EP1332Y]; RRID: AB_869989 |
Citations
(2) Datasheet |
Rabbit | Cytoplasmic Fraction | Size | 2.7 μg/mL | 54 |
| α-Tubulin | Primary |
MilliporeSigma MABT205 |
Tubulin alpha-1A chain; RRID: AB_11204167 |
Citations
(1) Datasheet |
Mouse | T-47D | Size | ||
| α2δ-1 | Primary |
MilliporeSigma D219 |
Dihydropyridine Receptor; RRID: AB_1078663 | Datasheet | Mouse | Brain homogenate | Size | 1:10, 1:50, 1:250 | 171 |
| α7nAChR | Primary |
Abcam ab216485 |
RRID: AB_3711367 |
Citations
(1) Datasheet |
Rabbit | Airway Smooth Muscle (ASM) | 1:50 | ||
| β-Actin | Primary |
Abcam ab179467 |
actin; RRID: AB_2737344 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
| β-Actin | Primary |
Abcam ab49900 |
beta (β) Actin ; RRID: AB_867494 |
Citations
(1) Datasheet |
Mouse | 1:100 | |||
| β-Actin | Primary |
ABclonal AC004 |
beta (β) Actin ; RRID: AB_2737399 |
Citations
(1) Datasheet |
Mouse | Phf2 KO myoblasts | 1:50 | ||
| β-Actin | Primary |
Cell Signaling Technology 5125 |
beta (β) Actin ; RRID: AB_1903890 |
Citations
(2) Datasheet |
Rabbit | Frozen samples | 1:2000 | ||
| β-Actin | Primary |
Abcam ab124964 |
alpha (α) smooth muscle Actin [EPR5368]; RRID: AB_11129103 |
Citations
(2) Datasheet |
Rabbit | Human Umbilical Vein Endothelial Cells (HUVECs) | 1:400 | ||
| β-actin | Primary |
Applied Biological Materials G043 |
beta-actin |
Citations
(1) Datasheet |
Mouse | MSCs cell lysate | Size | 37 | |
| β-Actin | Primary |
ABclonal AC026 |
RRID: AB_2768234 |
Citations
(3) Datasheet |
Rabbit | Pancreas | Size | 1:50 | |
| β-Actin | Primary |
Wanleibio WL01372 |
beta (β) Actin ; RRID: AB_2847841 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
| β-Actin | Primary |
Abcam ab213262 |
beta (β) Actin ; RRID: AB_2924820 |
Citations
(1) Datasheet |
Rabbit | Liver tissue homogenate (mouse) | |||
| β-Actin | Primary |
PTM BioLab PTM-5018 |
beta (β) Actin; RRID: AB_3086678 |
Citations
(1) Datasheet |
Mouse | Retinal tissue | Size | 1:50 | |
| β-Actin | Primary |
Proteintech 81115-1-RR |
beta (β) Actin; RRID: AB_2923704 |
Citations
(1) Datasheet |
Rabbit | Supernatant from lysate | |||
| β-Actin | Primary |
Bioss bs-0061R |
beta (β) Actin; RRID: AB_10855480 |
Citations
(1) Datasheet |
Rabbit | Rat kidney, pancreas, and heart | Size | 1:25, 1:75, 1:100, 1:150, 1:300 | 48 |
| β-Actin | Primary |
Proteintech 20536-1-AP |
beta (β) Actin ; RRID: AB_10700003 |
Citations
(3) Datasheet |
Rabbit | Protein extract from tissues | 1:200 | ||
| β-Actin | Primary |
Abcam ab115777 |
beta (β) Actin antibody [SP124]; RRID: AB_10899528 |
Citations
(1) Datasheet |
Rabbit | Vascular smooth muscle cells (VSMCs) | 1:150 | ||
| β-Amyloid | Primary |
Cell Signaling Technology 8243 |
Amyloid-beta precursor protein; RRID: AB_2797642 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:2000 | |
| β-amyloid | Primary |
Santa Cruz Biotechnology sc-28365 |
beta (β) Amyloid; RRID: AB_626669 |
Citations
(2) Datasheet |
Mouse | Fibroblasts, Mouse brain | Size | 1:50 | |
| β-catenin | Primary |
Santa Cruz Biotechnology sc-7963 |
Raised against amino acids 680-781 mapping at the C-terminus of human b-Cat; RRID: AB_626807 |
Citations
(1) Datasheet |
Mouse | Lung cancer cell | Size | 1:50 | |
| β-catenin | Primary |
ZenBio 201328-5D6 |
Citations
(1) Datasheet |
Mouse | Intestinal tissue | ||||
| β-Catenin | Primary |
Cell Signaling Technology 2951 |
Catenin, beta; RRID: AB_1196644 |
Citations
(1) Datasheet |
Total protein extracted (KYSE70 cells) | 1:50 | |||
| β-Tubulin | Primary |
MilliporeSigma T8328 |
RRID: AB_1844090 |
Citations
(1) Datasheet |
Mouse | Size | |||
| βIII Tubulin | Primary |
Abcam ab78078 |
Citations
(1) Datasheet |
Mouse Spinal Cord Homogenates | Size | 50 | |||
| γ-Actin | Primary |
GeneTex GTX101794 |
Actin γ 1; RRID: AB_2036153 |
Citations
(1) Datasheet |
Rabbit | Human Stomach Smooth Muscles | Size | 1:100 | 44 |
| γ-Tubulin | Primary |
MilliporeSigma T6557 |
Tubulin, gamma; RRID: AB_477584 |
Citations
(2) Datasheet |
Mouse | Cell lysates | 8 µg/mL | ||
| 14-3-3 β/α | Primary |
Cell Signaling Technology 9636 |
Unmodified; RRID: AB_560823 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 β Discontinued by vendor |
Primary |
Invitrogen Antibodies PA1-37002 |
RRID: AB_2217649 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:1000 | |
| 14-3-3 ε | Primary |
Cell Signaling Technology 9635 |
Unmodified; RRID: AB_2217758 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 η | Primary |
Cell Signaling Technology 9640 |
Unmodified; RRID: AB_2218073 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
Unmodified; RRID: AB_3276333 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | 34 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
Unmodified; RRID: AB_3275422 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 33 |
| 14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
Unmodified; RRID: AB_10002615 | Datasheet | Mouse | HeLa lysate 1.0 mg/mL | Size | 1:12.5 | 36 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-81016 |
RRID: AB_3410086 | Datasheet | Mouse | 1:12.5 | |||
| 14-3-3 γ | Primary |
Cell Signaling Technology 9637 |
Unmodified; RRID: AB_2218254 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 τ | Primary |
Cell Signaling Technology 9638 |
Unmodified; RRID: AB_2218251 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 ζ | Primary |
R&D Systems MAB2669 |
Unmodified; RRID: AB_2814945 | Datasheet | Mouse | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
| 14-3-3 ζ/δ | Primary |
Cell Signaling Technology 9639 |
Unmodified; RRID: AB_2273196 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 (pan) | Primary |
Cell Signaling Technology 8312 |
Pan; RRID: AB_10860606 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 (pan) | Primary |
Santa Cruz Biotechnology sc-1657 |
Pan; RRID: AB_626618 | Datasheet | Mouse | Mouse brain lysates | Size | 1:10, 1:50, 1:100 | 33 |
| 14-3-3 gamma (KC21)* | Primary |
OriGene TA301660 |
Datasheet | Mouse | HeLa | Size | 1:12.5 | 35 |