Expression of Wilms’ tumor gene (WT1) is found in normal tissues, but overexpression of WT1 is a common feature in various cancers, as its protein product is a potent transcriptional regulator acting as a tumor suppressor. Antibody-based detection of transcription factors has been historically difficult due to the rigid structure of protein-protein interactions, which may mask unidentified structural configurations. The RNAscope™ RNA in situ hybridization platform, including chromogenic and fluorescent detection reagents and target-specific probes, allows for the detection of WT1 at the single mRNA molecule level in individual cells, all while providing spatial and morphological context. The double Z probe design allows for simultaneous signal amplification and suppression of non-specific background staining, ensuring target-specific detection.
