Summary
This comprehensive reference guide presents guidelines, protocols, and data examples for obtaining optimal RNA in situ hybridization results with any tissue type from any species.
- The RNAscope™ workflow
- The importance of controls
- Choosing appropriate control probes
- Materials and methods
- Optimal pretreatment conditions for different tissues in different species
In addition, this eBook contains detailed discussions and recommendations related to:
Optimizing results by species and tissues
- Comparison of mild and standard pretreatment conditions
- Positive and negative control probe data
- Proliferation and apoptosis marker analysis
Troubleshooting
- Sample preparation and optimization
- Unexpected staining patterns
- High background
- Tissue detachment
- Other issues
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