VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit, R.T.U. (Ready to Use) HRP Immunodetection Kit
Novus Biologicals, part of Bio-Techne | Catalog # PK-7200-NB
Key Product Details
Species
Mouse, Rabbit
Applications
ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Kit Type
HRP Immunodetection Kit
Product Summary
Features:
Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
Available without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodies
Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.
Product Specifications
Application Notes
Contains sufficeint reagent to stain approximately 250-500 sections.
Note:ur Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species..
Note:ur Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species..
Kit Contents
- 50 ml 2.5% normal horse serum
- 50 ml ready-to-use stabilized Elite(R) ABC reagent
- 50 ml ready-to-use universal biotinylated anti-mouse/rabbit IgG
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C. Do not freeze.
Background: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit, R.T.U. (Ready to Use)
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)
Additional VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit, R.T.U. (Ready to Use) Products
Product Specific Notices
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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