RepSox

Product Description

RepSox is a potent and selective inhibitor of the TGF-β type I receptor/ALK5 (IC₅₀ values are 4 and 23 nM for TGF-β type I receptor autophosphorylation and binding respectively). RepSox is selective for TGF-β type I receptor over a range of kinases, including p38 MAPK, JNK1 and GSK3 (IC₅₀ > 16 μM). Enhances the efficiency of cellular reprogramming (see our protocols below); also used in protocols to generate CiPSCs from MEFs or β cells from hPSCs (see below); used in protocols to generate pancreatic beta cells from hPSCs or CiPSCs from MEFs. Replaces Sox2 by inducing Nanog expression.

RepSox synthesized to Ancillary Material Grade also available.

For more information about how RepSox may be used, see our protocols: Highly Efficient Generation of CiPSCs from MEFs, Generation of β cells from hPSCs, Reprogramming MEFs into Skeletal Muscle Progenitors.

Solubility

Preparing Stock Solutions for RepSox

The following data is based on the product molecular weight 287.32

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

10 9 8 7 6 5 4 3 2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.48 mL	17.40 mL	34.80 mL
5 mM	0.70 mL	3.48 mL	6.96 mL
10 mM	0.35 mL	1.74 mL	3.48 mL
50 mM	0.07 mL	0.35 mL	0.70 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Li Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 2009 PMID: 19097958
• Ichida A small-molecule inhibitor of Tgf-β signaling replaces Sox2 in reprogramming by inducing Nanog. Cell Stem Cell 2009 PMID: 19818703
• Gellibert Identification of 1,5-naphthyridine derivatives as a novel series of potent and selective TGF-γ type I receptor inhibitors. J.Med.Chem. 2004 PMID: 15317461