JB 300

Name: JB 300
Brand: Tocris Bioscience
SKU: 7837

Tocris Bioscience, part of Bio-Techne | Catalog # 7837

COA / SDS

Aurora A Degrader (PROTAC®)

Key Product Details

Description:	Aurora A Degrader (PROTAC^®)
Chemical Name:	trans-4-(3-Chloro-2-fluorophenoxy)-N-(2-(2-(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethoxy)ethoxy)ethyl)-1-((6-(thiazol-2-ylamino)pyridin-2-yl)methyl)cyclohexane-1-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	906.38

View Full Technical Details for JB 300

Technical Data
Citations

Biological Activity for JB 300

JB 300 is a potent and selective Aurora A Degrader (PROTAC^®; DC₅₀ = 30 nM and D_max = 78%). It comprises a cereblon E3-ligase ligand Thalidomide (Cat. No. 0652) joined by a PEG2 linker to the Aurora A inhibitor MK-5108. The compound depletes Aurora A in MV4-11 cell lines and exhibits no degradation of Aurora B at 10 µM. JB 300 shows dose-dependent cytotoxicity in AML cells.

Aurora A antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF3295 and NBP1-51843.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold under license from University of Würzburg

Technical Data for JB 300

M. Wt	906.38
Formula	C₄₃H₄₅ClFN₇O₁₀S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	3038446-90-8
InChi Key	LAZHTWDJEQSMOY-NKEKBGIASA-N
Smiles	O=C([C@@]1(CC2=CC=CC(NC3=NC=CS3)=N2)CC[C@H](OC4=C(F)C(Cl)=CC=C4)CC1)NCCOCCOCCNC(COC5=C6C(N(C7CCC(NC7=O)=O)C(C6=CC=C5)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JB 300

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	18.13	20

Preparing Stock Solutions for JB 300

The following data is based on the product molecular weight 906.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	5.52 mL	27.58 mL	55.16 mL
1 mM	1.10 mL	5.52 mL	11.03 mL
2 mM	0.55 mL	2.76 mL	5.52 mL
10 mM	0.11 mL	0.55 mL	1.10 mL

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References for JB 300

References are publications that support the biological activity of the product.

Bozolovic Novel, highly potent PROTACs targeting AURORA-A kinase. Curr.Res.Chem.Biol.
Adhikari PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase. Nat.Chem.Biol. 2020 PMID: 32989298