• 6 months from date of receipt, -20 to -70 °C as supplied. • 3 months, -20 to -70 °C under sterile conditions after opening. • Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Applications
Used as a ligand for lectins and to study glycan protein interaction.
Used as a substrate for various glycosidase and glycosyltransferases.
Used as a substrate fucosidase activity and specificity studies.
Key Features:
Excitation at 649 nm and emission at 671 nm.
The fluorescent dye Cy5 is conjugated to the C6 position of the core-6 fucose.
Can be separated on 15-17% SDS-PAGE and directly visualized as a single band through the red channel of a fluorescent imager.
Linear response range for Cy5-labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.
Product Specifications for Cy5-Fuc Labeled Lewis X (Cy5-Lewis X)
Source
Synthetic
Preparation & Storage
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Assay Procedure
Sample Assay Protocol for testing activity of Fucosidase using Cy5-Fuc Labeled Lewis X/Cy5-Lewis X as a substrate.
Suggested input of Cy5-Lewis X in an assay separated on SDS-PAGE is from 0.01-1 pmol.
Protocols are guidelines. Parameters need to be optimized by end users, enzyme input and reaction time may need to be adjusted to accommodate specific activity of the enzyme.
