Ch 55

Product Description

Ch 55 is a highly potent synthetic retinoid that has high affinity for RAR-α and RAR-β receptors and low affinity for cellular retinoic acid binding protein (CRABP). Inhibits rabbit tracheal epithelial cell differentiation by inhibiting transglutaminase and increasing cholesterol sulfate (EC₅₀ values are 0.02 and 0.03 nM respectively). Induces differentiation of embryonic carcinoma F9 and melanoma S91 cells (EC₅₀ values are 0.26 and 0.5 nM respectively) and inhibits the induction of ornithine decarboxylase activity in 3T6 fibroblasts (EC₅₀ = 1 nM). Enables generation of Chemically Induced Pluripotent Stem Cells (CiPSCs) from mouse embryonic fibroblasts (MEFs) (see our protocol below).

For more information about how Ch 55 may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs

Solubility

Preparing Stock Solutions for Ch 55

The following data is based on the product molecular weight 364.47

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.74 mL	13.72 mL	27.44 mL
5 mM	0.55 mL	2.74 mL	5.49 mL
10 mM	0.27 mL	1.37 mL	2.74 mL
50 mM	0.05 mL	0.27 mL	0.55 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Sato Functional studies of newly sythesized benzoic acid derivatives: identification of highly potent retinoid-like activity. J.Cell.Physiol. 1988 PMID: 2836439
• Hashimoto Expression of retinoic acid receptor genes and the ligand-binding selectivity of retinoic acid receptors (RAR's). Biochem.Biophys.Res.Comm.
• Jetten New benzoic acid derivatives with retinoid activity: lack of direct correlation between biological activity and binding to cellular retinoic acid binding protein. Cancer Res. 1987 PMID: 2884032