Auxinole
Tocris Bioscience, part of Bio-Techne | Catalog # 7894
Auxin antagonist of TIR1/AFB receptors; also OsTIR1 inhibitor in auxin-inducible degron (AID) system
Key Product Details
Description
Auxin antagonist of TIR1/AFB receptors; also OsTIR1 inhibitor in auxin-inducible degron (AID) system
Product Description
Auxinole is an auxin antagonist of TIR1/AFB receptors. Binds to TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and inhibit auxin-responsive gene expression. Auxinole competitively inhibits various auxin responses in planta. Auxinole also inhibits OsTIR1 that suppresses leaky degradation of degron-fused proteins. Can be used as a control compound with 5-Ph-IAA (Cat. No. 7392) in the AID2 degron system to switch off degradation.Product Specifications
Molecular Weight
321.37
Formula
C20H19NO3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
α-[2-(2,4-Dimethylphenyl)-2-oxoethyl]-1H-indole-3-acetic acid
CAS Number
86445-22-9
PubChem ID
13077496
InChI Key
HGUYAIJBXSQXGV-UHFFFAOYSA-N
SMILES
O=C(O)C(C1=CNC=2C=CC=CC21)CC(=O)C3=CC=C(C=C3C)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.14 | 100 | |
| Ethanol | 6.43 | 20 with gentle warming |
Preparing Stock Solutions
for Auxinole
The following data is based on the product molecular weight 321.37
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.11 mL | 15.56 mL | 31.12 mL |
| 5 mM | 0.62 mL | 3.11 mL | 6.22 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.11 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Yesbolatova Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods 2019 PMID: 31026591
- Hayashi Rational design of an auxin antagonist of the SCF(TIR1) auxin receptor complex. ACS Chem.Biol. 2012 PMID: 22234040
Product Specific Notices for Auxinole
For research use only
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