5-Ph-IAA
Tocris Bioscience, part of Bio-Techne | Catalog # 7392
Selective and potent TAG Degrader for auxin-inducible degron 2 system
Key Product Details
Description
Selective and potent TAG Degrader for auxin-inducible degron 2 system
Product Description
5-Ph-IAA is a selective and potent "Bump & Hole" TAG Degrader (DC50,6h = 17.0 nM, T1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system. 5-Ph-IAA is an analog (the “Bump”) of natural auxin that induces rapid degradation of a target protein tagged with mAID F-box protein (the "Hole" or mini AID), and recruits a mutant E3 ligase complex, OsTIR1(F74G). The induced degradation is reversible 3 h after washout with fresh medium. 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system. The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo.5-Ph-IAA-AM (Cat. No. 7893), an analog of 5-Ph-IAA, is also available.
Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1F74G and mAID tag are available from Addgene.
Product Specifications
Molecular Weight
251.29
Formula
C16H13NO2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
5-Phenyl-1H-indole-3-acetic acid
CAS Number
168649-23-8
PubChem ID
15207491
InChI Key
ACCCWYHBDGKDKN-UHFFFAOYSA-N
SMILES
OC(CC1=CNC2=C1C=C(C3=CC=CC=C3)C=C2)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.13 | 100 | |
| Ethanol | 25.13 | 100 |
Preparing Stock Solutions
for 5-Ph-IAA
The following data is based on the product molecular weight 251.29
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.98 mL | 19.90 mL | 39.79 mL |
| 5 mM | 0.80 mL | 3.98 mL | 7.96 mL |
| 10 mM | 0.40 mL | 1.99 mL | 3.98 mL |
| 50 mM | 0.08 mL | 0.40 mL | 0.80 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Yesbolatova The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat.Commun. 2020 PMID: 33177522
- Zhang An improved auxin-inducible degron system for fission yeast. G3 Genes|Genomes|Genetics 2021 PMID: 34849776
- Yeung One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines. Mol.Biol.Cell 2021 PMID: 33979199
Product Specific Notices for 5-Ph-IAA
For research use only
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