AMG 333

Name: AMG 333
Brand: Tocris Bioscience
SKU: 6874

Tocris Bioscience, part of Bio-Techne | Catalog # 6874

Citations (5)

COA / SDS

Potent and selective TRPM8 antagonist; active in vivo

Key Product Details

Description:	Potent and selective TRPM8 antagonist; active in vivo
Chemical Name:	6-[[[(S)-(3-Fluoro-2-pyridinyl)[3-fluoro-4-(trifluoromethoxy)phenyl]methyl]amino]carbonyl]-3-pyridinecarboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	453.33

View Full Technical Details for AMG 333

Technical Data
Citations

Biological Activity for AMG 333

AMG 333 is a potent and selective TRPM8 antagonist (IC₅₀ values are 13 and 20 nM for hTRPM8 and rTRPM8, respectively). Displays high selectivity over other TRP channels (IC₅₀ >20 μM, TRPV1/V3/V4/A1). Inhibits icilin-induced wet-dog shake in rats and suppresses cold-induced increases in blood pressure in the rat cold-pressor test.

Compound Libraries for AMG 333

AMG 333 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for AMG 333

M. Wt	453.33
Formula	C₂₀H₁₂F₅N₃O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1416799-28-4
PubChem ID	71144018
InChi Key	QEBYISWYMFIXOZ-INIZCTEOSA-N
Smiles	OC(=O)C1=CC=C(N=C1)C(=O)N[C@@H](C1=CC(F)=C(OC(F)(F)F)C=C1)C1=C(F)C=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AMG 333

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	45.33	100
	ethanol	45.33	100

Preparing Stock Solutions for AMG 333

The following data is based on the product molecular weight 453.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.21 mL	11.03 mL	22.06 mL
5 mM	0.44 mL	2.21 mL	4.41 mL
10 mM	0.22 mL	1.10 mL	2.21 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for AMG 333

References are publications that support the biological activity of the product.

Horne Discovery of TRPM8 antagonist (S)-6-(((3-Fluoro-4-(trifluoromethoxy)phenyl)(3-fluoropyridin-2-yl)methyl)carbamoyl)nicotinic acid (AMG 333), a clinical candidate for the treatment of migraine. J.Med.Chem. 2018 PMID: 30148953