Icilin

Name: Icilin
Brand: Tocris Bioscience
SKU: 1531

Tocris Bioscience, part of Bio-Techne | Catalog # 1531

Citations (47)

COA / SDS

Activates cold receptors. Cooling agent

Key Product Details

Description:	Activates cold receptors. Cooling agent
Alternative Names:	AG 3-5
Chemical Name:	3,4-Dihydro-3-(2-hydroxyphenyl)-6-(3-nitrophenyl)-(1H)-pyrimidin-2-one
Purity:	≥98% (HPLC)
Molecular Weight:	311.3

View Full Technical Details for Icilin

Technical Data
Citations

Biological Activity for Icilin

Icilin is a cooling agent that activates the novel cold receptors TRPM8 (CMR1) and TRPA1 (ANKTM1/TRPN1), members of the TRP ion channel family. Induces currents in CMR1-expressing HEK 293 cells (EC₅₀ = 0.36 μM) more potently than menthol or low temperatures. Produces "wet shakes" in vivo.

Technical Data for Icilin

M. Wt	311.3
Formula	C₁₆H₁₃N₃O₄
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	36945-98-9
PubChem ID	161930
InChi Key	RCEFMOGVOYEGJN-UHFFFAOYSA-N
Smiles	OC1=C(C=CC=C1)N1CC=C(NC1=O)C1=CC=CC(=C1)[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Icilin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	31.13	100

Preparing Stock Solutions for Icilin

The following data is based on the product molecular weight 311.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.21 mL	16.06 mL	32.12 mL
5 mM	0.64 mL	3.21 mL	6.42 mL
10 mM	0.32 mL	1.61 mL	3.21 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

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References for Icilin

References are publications that support the biological activity of the product.

Diver Science.
Wei AG-3-5: a chemical producing sensations of cold. J.Pharm.Pharmacol. 1983 PMID: 6131976
McKemy Identification of a cold receptor reveals a general role for TRP channels in thermosensation. Nature 2002 PMID: 11882888
Story ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell 2003 PMID: 12654248
Behrendt Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay. Br.J.Pharmacol. 2004 PMID: 14757700