Actinomycin D
Tocris Bioscience, part of Bio-Techne | Catalog # 1229
Inhibits RNA polymerase
Product Description
Actinomycin D is an anti-neoplastic antibiotic. Inhibits RNA polymerase and is a potent inducer of apoptosis.Product Specifications
Molecular Weight
1255.43
Formula
C62H86N12O16
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-Amino-(N,N)-1-bis(hexadecahydro-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-1H-pyrrolo[2,1]-[1,4,7,10,13] oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
CAS Number
50-76-0
PubChem ID
2019
InChI Key
RJURFGZVJUQBHK-DCUCITLNSA-N
SMILES
[H][C@@]12CCCN1C([C@H](C(C)C)NC([C@H]([C@H](OC([C@@H](N(C(CN(C2=O)C)=O)C)C(C)C)=O)C)NC(C3=C4N=C5C(OC4=C(C=C3)C)=C(C(C(N)=C5C(N[C@H]6[C@H](OC([C@@H](N(C(CN(C([C@@]7(CCCN7C([C@H](C(C)C)NC6=O)=O)[H])=O)C)=O)C)C(C)C)=O)C)=O)=O)C)=O)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 62.77 | 50 |
Preparing Stock Solutions
for Actinomycin D
The following data is based on the product molecular weight 1255.43
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 1.59 mL | 7.97 mL | 15.93 mL |
| 2.5 mM | 0.32 mL | 1.59 mL | 3.19 mL |
| 5 mM | 0.16 mL | 0.80 mL | 1.59 mL |
| 25 mM | 0.03 mL | 0.16 mL | 0.32 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Glynn Apoptosis induced by actinomycin D, camptothecin or aphidicolin can occur in all phases of the cell cycle. Biochem.Soc.Trans. 1992 PMID: 1634006
- Jeeninga The mechanism of actinomycin D-mediated inhibition of HIV-1 reverse transcription. Nucleic Acids Res. 1998 PMID: 9826774
- Aktipis A kinetic study on the mechanism of inhibition of RNA synthesis catalyzed by DNA-dependent RNA polymerase. Differences in inhibition by ethidium bromide, 3,8-diamino-6-ethylphenanthridinium bromide and actinomycin D. Biochim.Biophys.Acta 1981 PMID: 7025910
Product Specific Notices for Actinomycin D
For research use only
⚠ WARNING: This product can expose you to chemicals including Actinomycin D, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov
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