Simple Plex Human TGF-beta 1 (native) Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002754
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TGF-beta 1 (native) Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 76.9 | 3964 | 76.0 | 3820 |
| Standard Deviation | 4.14 | 242 | 7.60 | 428 |
| CV% | 5.4 | 6.1 | 10.0 | 11.2 |
Recovery for Simple Plex Human TGF-beta 1 (native) Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Platelet-poor EDTA Plasma (n=4) | 105 | 98-112 |
| Platelet-poor Heparin Plasma (n=4) | 99 | 82-111 |
| Serum (n=4) | 92 | 89-99 |
Linearity
Samples containing and/or spiked with high concentrations of human TGF-beta 1 (native) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human TGF-beta 1 (native) Cartridge
Human TGF-beta 1 (native) Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: TGF-beta 1
TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.
Long Name
Alternate Names
Gene Symbol
Additional TGF-beta 1 Products
Product Documents for Simple Plex Human TGF-beta 1 (native) Cartridge
Product Specific Notices for Simple Plex Human TGF-beta 1 (native) Cartridge
For research use only