Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge

Name: Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-004314

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-004314

Product Datasheet / COA

Simple Plex Cartridge Kit for quantitation of OPN. Contains cartridge, sample diluent SD13, and wash buffer

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	7.33 pg/mL
Assay Range:	20.97 - 12,800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge Summary:	Simple Plex assay is for the detection of human Osteopontin (OPN) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human OPN.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one OPN assay

Inter-Assay Precision (Precision between assays) Replicates of each OPN control were tested in multiple assays performed by at least three technicians using two lots of reagents for human OPN

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	42	42
Mean (pg/mL)	221	10403	93.2	4790
Standard Deviation	16.19	661	9.46	454
CV%	7.3	6.4	10.1	9.5

Linearity

Samples containing and/or spiked with high concentrations of human OPN were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge

	Simple Plex Human OPN Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human OPN Assay. Assay range is 21.0-12,800 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Item	Value
Long Name	Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names	BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, Osteopontin, SPP-1, SPP1/CALPHA1 fusion, Spp1, Urinary stone protein, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), uropontin

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Product Documents for Simple Plex Human Osteopontin/OPN 2nd Gen Cartridge

Product Datasheets

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COA

Protocols & Manuals

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