Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-014625
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one OX40 Ligand assay.
Inter-Assay Precision (Precision between assays) Replicates of each OX40 Ligand control were tested in multiple assays performed by at least three technicians using two lots of reagents for human OX40 Ligand.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 32 | 33 |
| Mean (pg/mL) | 156 | 7307 | 92.5 | 3910 |
| Standard Deviation | 11.9 | 475 | 8.00 | 335 |
| CV% | 7.6 | 6.5 | 8.7 | 8.6 |
Recovery for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay
Recovery for the human OX40 Ligand assays was evaluated at three different spiked concentrations of OX40 Ligand protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 91-117 |
| EDTA Plasma (n=2) | 85 | 74-95 |
| Heparin Plasma (n=2) | 100 | 95-104 |
| Serum (n=2) | 90 | 86-95 |
Linearity
Samples containing and/or spiked with high concentrations of OX40 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay
Simple Plex Discovery Human OX40 Ligand Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human OX40 Ligand Assay. Assay range is 3.14-12,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: OX40 Ligand/TNFSF4
OX40 Ligand (OX40L), also known as gp34, is a member of the TNF superfamily. Human OX40L shares 46% amino acid sequence identity with its mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similar to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily, which is expressed predominantly on activated CD4+ T cells.
Alternate Names
Gene Symbol
Additional OX40 Ligand/TNFSF4 Products
Product Documents for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay
Product Specific Notices for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay
For research use only