Recombinant Human FKBP14 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45269

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all FKBP14 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-211 of Human FKBP14

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 240 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FKBP14 His Protein

SDS-PAGE: Recombinant Human FKBP14 His Protein [NBP1-45269]

SDS-PAGE: Recombinant Human FKBP14 His Protein [NBP1-45269]

SDS-Page: FKBP14 Recombinant Protein [NBP1-45269]

Formulation, Preparation and Storage

NBP1-45269
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FKBP14

FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus. Recombinant human FKBP14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography

Alternate Names

22 kDa FK506-binding protein, EC 5.2.1.8, FK506 binding protein 14 (22 kDa), FK506 binding protein 14, 22 kDa, FK506-binding protein 14, FKBP-14, FKBP-22, FKBP22PPIase FKBP14, FLJ20731, peptidyl-prolyl cis-trans isomerase FKBP14,22 kDa FKBP, Rotamase

Gene Symbol

FKBP14

Additional FKBP14 Products

Product Documents for Recombinant Human FKBP14 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human FKBP14 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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