Recombinant Human Aspartate Aminotransferase His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52179
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Enzyme Activity, SDS-PAGE
Product Specifications
Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-413 of Human Aspartate Aminotransferase
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
Purity
>95%, by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Predicted Molecular Mass
48.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Aspartate Aminotransferase His Protein
![SDS-PAGE: Recombinant Human Aspartate Aminotransferase His Protein [NBP2-52179]](https://resources.bio-techne.com/images/products/Aspartate-Aminotransferase-Recombinant-Protein-SDS-Page-NBP2-52179-img0001.jpg "SDS-PAGE: Recombinant Human Aspartate Aminotransferase His Protein [NBP2-52179]")
SDS-PAGE: Recombinant Human Aspartate Aminotransferase His Protein [NBP2-52179]
SDS-Page: Recombinant Human Aspartate Aminotransferase Protein [NBP2-52179] - 15% SDS Page (3 ug)Formulation, Preparation and Storage
NBP2-52179
| Formulation | 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 2 mM DTT, 100 mM NaCl |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Aspartate Aminotransferase
Long Name
Glutamic-oxaloacetic transaminase 1
Alternate Names
aspartate aminotransferase, cytoplasmic, AST1, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, SGOT, Transaminase A
Gene Symbol
GOT1
Additional Aspartate Aminotransferase Products
Product Documents for Recombinant Human Aspartate Aminotransferase His Protein
Product Specific Notices for Recombinant Human Aspartate Aminotransferase His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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