Recombinant Mouse Adenylate Kinase 1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61200

Novus Biologicals, part of Bio-Techne
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NBP2-61200-20ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Adenylate Kinase 1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-210 of Mouse Adenylate Kinase 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

25.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Adenylate Kinase 1 His Protein

SDS-PAGE: Recombinant Mouse Adenylate Kinase 1 His Protein [NBP2-61200]

SDS-PAGE: Recombinant Mouse Adenylate Kinase 1 His Protein [NBP2-61200]

SDS-Page: Recombinant Mouse Adenylate Kinase 1 Protein [NBP2-61200] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP2-61200
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Gene Symbol

AK1

Additional Adenylate Kinase 1 Products

Product Documents for Recombinant Mouse Adenylate Kinase 1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Adenylate Kinase 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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