Recombinant Human Adenylate Kinase 1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50855

Novus Biologicals, part of Bio-Techne
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NBP1-50855-0.1mg
NBP1-50855-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Adenylate Kinase 1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-194 of Human Adenylate Kinase 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Adenylate Kinase 1 His Protein

SDS-PAGE: Recombinant Human Adenylate Kinase 1 His Protein [NBP1-50855]

SDS-PAGE: Recombinant Human Adenylate Kinase 1 His Protein [NBP1-50855]

SDS-Page: Recombinant Human Adenylate Kinase 1 Protein [NBP1-50855]

Formulation, Preparation and Storage

NBP1-50855
Formulation 20 mM Tris-HCl buffer (pH 7.5), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Gene Symbol

AK1

Additional Adenylate Kinase 1 Products

Product Documents for Recombinant Human Adenylate Kinase 1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Adenylate Kinase 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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