Thioflavin X New

Name: Thioflavin X
Brand: Tocris Bioscience
SKU: 8949

Tocris Bioscience, part of Bio-Techne | Catalog # 8949

COA / SDS

Next generation cell-permeable fluorescent amyloid stain. Used for: in vitro Aβ staining in brain tissues.

Key Product Details

Description:	Next generation cell-permeable fluorescent amyloid stain. Used for: in vitro Aβ staining in brain tissues.
Chemical Name:	6-Methoxy-3-methyl-2-(4-(pyrrolidin-1-yl)phenyl)benzo[d]thiazol-3-ium iodide
Purity:	≥95% (HPLC)
Molecular Weight:	452.35

View Full Technical Details for Thioflavin X

Technical Data
Citations

Biological Activity for Thioflavin X

Key information: Thioflavin X (ThX) is a next-generation cell-permeable fluorescent probe for the detection of amyloid aggregates. Displays 5-fold increase in brightness and 7-fold increase in binding affinity (K_i = 680 nM) compared to Thioflavin T

Used for: staining of Aβ oligomers, P301S Tau and α-Syn aggregates in brain tissues

Application: fluorescence microscopy and super resolution microscopy

Properties and Photophysical Data: Thioflavin X increases in fluorescence upon binding to α-Syn aggregates. Excitation and emission maxima (λ) in the presence of α-Syn aggregates are 421 nm and 490 nm, respectively; quantum yield = 0.81; extinction coefficient = 38,114 M^-1cm^-1

Optical Data for Thioflavin X

λ_abs	421 nm
λ_em	490 nm
Extinction Coefficient (ε)	38114 M^-1cm^-1
Quantum Yield (φ)	0.81

Plan Your Experiments

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Technical Data for Thioflavin X

M. Wt	452.35
Formula	C₁₉H₂₁IN₂OS
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2683063-26-3
PubChem ID	170907366
InChi Key	IJDBRVINIKHPDK-UHFFFAOYSA-M
Smiles	C[N+]1=C(C2=CC=C(N3CCCC3)C=C2)SC4=CC(OC)=CC=C41.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Thioflavin X

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	45.23	100

Preparing Stock Solutions for Thioflavin X

The following data is based on the product molecular weight 452.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.21 mL	11.05 mL	22.11 mL
5 mM	0.44 mL	2.21 mL	4.42 mL
10 mM	0.22 mL	1.11 mL	2.21 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for Thioflavin X

References are publications that support the biological activity of the product.

Needham ThX - a next-generation probe for the early detection of amyloid aggregates. Chem.Sci. 2020 PMID: 34122915