Thioflavin T

Name: Thioflavin T
Brand: Tocris Bioscience
SKU: 7122

Tocris Bioscience, part of Bio-Techne | Catalog # 7122

Citations (1)

COA / SDS

Cell-permeable fluorescent amyloid stain. Used for: in vitro Aβ staining in brain tissues.

Key Product Details

Description:	Cell-permeable fluorescent amyloid stain. Used for: in vitro Aβ staining in brain tissues.
Chemical Name:	2-[4-(Dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
Purity:	≥95% (HPLC)
Molecular Weight:	318.86

View Full Technical Details for Thioflavin T

Technical Data
Citations

Biological Activity for Thioflavin T

Key information: Thioflavin T is a cell-permeable fluorescent amyloid stain.

Used for: staining of insoluble senile plaques of Aβ in brain tissues, monitoring the kinetics of in vitro polyglutamine amyloid formation of tNhtt-42Q aggregates in a Huntington's disease cell model. Thioflavin T is used to confirm formation of β sheet structure from mutant huntingtin exon-1 aggregates (Ex1Q48) in vitro.

Application: fluorescence microscopy.

Properties and Photophysical Data: Thioflavin T is a cationic benzothiazole. It increases in fluorescence upon strong binding (K_i = 580 nM) to the stacked β sheets of amyloid fibrils. Excitation and emission maxima (λ) are 440 nm and 485 nm, respectively; quantum yield = 0.43; extinction coefficient = 31600 M^-1cm^-1.

This product is supplied with a high degree of hydration and some residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Optical Data for Thioflavin T

Emission Color	Blue
λ_abs	440 nm
λ_em	485 nm
Extinction Coefficient (ε)	31600 M^-1cm^-1
Quantum Yield (φ)	0.43
Cell Permeable	Yes
Application	Amyloid Stain

Technical Data for Thioflavin T

M. Wt	318.86
Formula	C₁₇H₁₉ClN₂S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2390-54-7
PubChem ID	16953
InChi Key	JADVWWSKYZXRGX-UHFFFAOYSA-M
Smiles	CC1=CC2=C([N+](C)=C(C3=CC=C(N(C)C)C=C3)S2)C=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Thioflavin T

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	1.59	5
	water	3.19	10

Preparing Stock Solutions for Thioflavin T

The following data is based on the product molecular weight 318.86.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.36 mL	156.81 mL	313.62 mL
0.5 mM	6.27 mL	31.36 mL	62.72 mL
1 mM	3.14 mL	15.68 mL	31.36 mL
5 mM	0.63 mL	3.14 mL	6.27 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Thioflavin T

References are publications that support the biological activity of the product.

Lee Fluorescence chemicals to detect insoluble and soluble amyloid-β aggregates. ACS Chem.Neurosci. 2019 PMID: 31009195
Schindler Small, seeding-competent huntingtin fibrils are prominent aggregate species in brains of zQ175 Huntington's disease knock-in mice. Front.Neurosci. 2021 PMID: 34239412
Doi RNA-binding protein TLS is a major nuclear aggregate-interacting protein in huntingtin exon 1 with expanded polyglutamine-expressing cells. J.Biol.Chem. 2008 PMID: 18167354