Janelia Fluor® 635, BromoCatch™ Ligand
Tocris Bioscience, part of Bio-Techne | Catalog # 8937
Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
Key Product Details
Description
Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
Product Description
Janelia Fluor® 635, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 635, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 635 dye, delivering bright, red-shifted signal with minimal background fluorescence. Janelia Fluor® 635, BromoCatch™ Ligand enables nuclear-targeted labeling of fusion constructs such as H2B-BromoCatch, and shows no off-target activity in wild-type or untransfected cells. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 635, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.Please refer to this protocol for further information on how to use this product.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product is part of an early adopter program.
BromoCatch™ is a trademark of Bio-Techne Corp.
Scientific Data Images for Janelia Fluor® 635, BromoCatch™ Ligand
In gel fluorescence of Janelia Fluor® 635, BromoCatch™ Ligand.
In gel assay titrating the different proteins with increasing concentrations of the Janelia Fluor® 635, BromoCatch™ Ligand probe.
Multiplexed fluorescence imaging of HEK293 cells expressing GFP-BromoCatch™ and H2B-HaloTag®.
Cells were labeled with Hoechst 33342 (Cat# 5117), Janelia Fluor® 635, BromoCatch™ Ligand (Cat# 8937), (1µM), and Janelia Fluor® 549, Haloalkane (Cat# 8806), (100 nM). Intrinsic GFP fluorescence provides an orthogonal channel confirming GFP-BromoCatch™ expression, demonstrating robust multichannel compatibility in live-cell imaging.Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of DundeeOptical Data
| λabs | 646 nm |
| λem | 663 nm |
| Cell Permeable | Yes |
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Product Specifications
Molecular Weight
1152.40
Formula
C61H67F2N9O8SSi
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
InChI Key
KUWXUURGPXZQEI-RYOPHROTSA-N
SMILES
CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C([Si](C)(C7=CC(N8CC(C8)F)=CC=C57)C)=C/C(C=C6)=[N+]9CC(C/9)F)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.52 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 635, BromoCatch™ Ligand
The following data is based on the product molecular weight 1152.40
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.68 mL | 43.39 mL | 86.78 mL |
| 0.5 mM | 1.74 mL | 8.68 mL | 17.36 mL |
| 1 mM | 0.87 mL | 4.34 mL | 8.68 mL |
| 5 mM | 0.17 mL | 0.87 mL | 1.74 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Rodriguez-Rios BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. Nat.Commun. 2026 PMID: 42128888
Product Specific Notices for Janelia Fluor® 635, BromoCatch™ Ligand
For research use only
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