HB-2-30
Tocris Bioscience, part of Bio-Techne | Catalog # 8831
Fluorescent TG2/LRP1 substrate for imaging endocytosis
Key Product Details
Description
Fluorescent TG2/LRP1 substrate for imaging endocytosis
Product Description
Key information: HB-2-30 is a far-red fluorescent transglutaminase-2 (TG2) probe for imaging endocytosis in vitro and in vivo. HB-2-30 undergoes highly efficient endocytosis in complex with TG2 and α2-macroglobulin via the LRP1 pathway.Used for: Imaging endocytosis in vitro and in vivo, explore targeted lysosomal degradation of secreted and cell surface proteins.
Application: Confocal microscopy.
Properties and Photophysical Data: Suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining. Excitation and emission maxima (λ) are 649 and 665 nm, respectively.
Scientific Data Images for HB-2-30
Application of HB-2-30 in vitro.
Confocal microscopy images of bone marrow-derived dendritic cells from WT C57BL/6 mice (left) and TG2KO mice (right) incubated with 3μM HB-2-30. Cells were incubated for 90 minutes with the probe before being fixed, stained for nuclei (blue), and imaged. HB-2-30 signals is in red. Image kindly provided by Fu-Chen Yang and Harrison Besser, University or Stanford.
Application of HB-2-30 in vivo.
Ileum tissue sections from mice dosed with 10 mg/kg HB-2-30. WT C57BL/6 mice (left) and TG2KO mice (right) were dosed with HB-2-30 and sacrificed after 2 hours. Intestines were Swiss rolled, thin sectioned, stained for nuclei (blue), and imaged via confocal fluorescence microscopy. HB-2-30 signal is in red. Image kindly provided by Fu-Chen Yang and Harrison Besser, University or Stanford.Licensing Information
Sold under license from Stanford UniversityOptical Data
| λabs | 649 nm |
| λem | 665 nm |
| Application | Confocal microscopy |
Spectra Viewer
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
1307.59
Formula
C66H86N10O14S2
Storage
Store at -20°C
Purity
≥90% (HPLC)
CAS Number
3031522-14-9
InChI Key
ILCHGSXJNAIGGG-AFAVFPMXSA-N
SMILES
O=C(N1[C@@H](CCC1)C(N[C@H](C(N)=O)CC2=CC=CC=C2)=O)[C@@H](NC([C@H](CC/C=C/C(N(C)C)=O)NC([C@H]3N(CCC3)C(C)=O)=O)=O)CCNC(CCCCC[N+]4=C(/C=C/C=C/C=C5C(C)(C6=CC(S(=O)(O)=O)=CC=C6N\5C)C)C(C)(C)C7=CC(S(=O)([O-])=O)=CC=C47)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble to 13.08mg/ml in DMSO |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Du Pré Peyer’s patch B cells sample transglutaminase-gluten complexes and drive celiac disease autoimmunity. Gastroenterology 2025 PMID: 40602545
- Loppinet Targeted lysosomal degradation of secreted and cell surface proteins through the LRP-1 pathway. J.Am.Chem.Soc. 2023 PMID: 37590164
- Yang A dendritic cell population responsible for transglutaminase 2-mediated gluten antigen presentation in celiac disease. JCI Insight. 2025 PMID: 40875488
- Loppinet LRP-1 links post-translational modifications to efficient presentation of celiac disease-specific T cell antigens. Cell Chem.Biol. 2023 PMID: 36608691
Product Specific Notices for HB-2-30
For research use only
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