Human t-Plasminogen Activator/tPA DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY7449-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
78.1-5000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human t-Plasminogen Activator/tPA DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human t-Plasminogen Activator/tPA DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human t-Plasminogen Activator (tPA). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, plasma, saliva, and urine samples. Diluents for complex matrices, such as serum, plasma, saliva, and urine, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human t-Plasminogen Activator/tPA DuoSet ELISA
Human t-Plasminogen Activator / tPA ELISA Standard Curve
Kit Contents for Human t-Plasminogen Activator/tPA DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: t-Plasminogen Activator/tPA
tPA (tissue-type Plasminogen Activator) is a serine protease that is secreted by vascular endothelial cells, fibroblasts, neurons, microglia, and astrocytes. The partially active single chain can be further processed to full activity by Plasmin, Kallikrein 1, or Coagulation Factor Xa. Active tPA converts Plasminogen to Plasmin, a fibrinolytic protease, by hydrolyzing an Arg-Val peptide bond in Plasminogen. Unusually high levels of tPA activity can result in excessive bleeding, and low levels of tPA activity can result in thrombosis or embolism. tPA-mediated breakdown of the extracellular is also involved in promoting synaptic plasticity, neurite outgrowth, and synapse remodeling. Human tPA contains an N-terminal fibronectin type-1 domain, an epidermal growth factor-like domain, two kringle domains, and a serine protease catalytic domain.
Long Name
Tissue Plasminogen Activator
Alternate Names
Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator
Gene Symbol
PLAT
Additional t-Plasminogen Activator/tPA Products
Product Documents for Human t-Plasminogen Activator/tPA DuoSet ELISA
Product Specific Notices for Human t-Plasminogen Activator/tPA DuoSet ELISA
For research use only
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