Human SPARC-like 1/SPARCL1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2728
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
46.9-3000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human SPARC-like 1/SPARCL1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human SPARC-like 1/SPARCL1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human SPARC-like 1 / SPARCL1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human SPARC-like 1/SPARCL1 DuoSet ELISA
Human SPARC-like 1 / SPARCL1 ELISA Standard Curve
Kit Contents for Human SPARC-like 1/SPARCL1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SPARC-like 1/SPARCL1
SPARC-L1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as Hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins. SPARC-L1 is a widely expressed protein with antiadhesive properties. It is thought to terminate neuronal migration by antagonizing adhesion, and promote extravasation in tonsil high endothelial venules by inducing endothelial cell dissociation. SPARC-L1 binds collagen and contributes to formation of dermal collagen fibrils.
Alternate Names
Hevin, MAST9, SC1, SPARC like 1, SPARCL1
Gene Symbol
SPARCL1
Additional SPARC-like 1/SPARCL1 Products
Product Documents for Human SPARC-like 1/SPARCL1 DuoSet ELISA
Product Specific Notices for Human SPARC-like 1/SPARCL1 DuoSet ELISA
For research use only
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