Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPLG70
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.284 ng/mL
Assay Range
0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit
The Quantikine Human PLA2G7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure PLA2G7 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human PLA2G7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PLA2G7 showed linear curves that were parallel to the curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PLA2G7.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PLA2G7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.69 | 3.52 | 11.7 | 2.35 | 4.34 | 13.0 |
| Standard Deviation | 0.115 | 0.192 | 0.272 | 0.226 | 0.300 | 0.676 |
| CV% | 6.8 | 5.5 | 2.3 | 9.6 | 6.9 | 5.2 |
Recovery for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit
The recovery of PLA2G7 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 92 | 85-105 |
| EDTA Plasma (n=4) | 101 | 91-112 |
| Heparin Plasma (n=4) | 102 | 92-106 |
| Serum (n=4) | 106 | 91-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PLA2G7 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit
Human PLA2G7/PAF-AH/Lp-PLA2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PLA2G7/PAF-AH/Lp-PLA2
Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.
Long Name
Phospholipase A2 Group VII
Alternate Names
LDL-PLA2, Lp-PLA2, LpPLA2, PAF-AH
Gene Symbol
PLA2G7
Additional PLA2G7/PAF-AH/Lp-PLA2 Products
Product Documents for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit
Product Specific Notices for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.