Polyamine Supplement x1000 (lyophilized)

Product Description

Polyamine Supplement x1000 (lyophilized) is a media supplement comprising a lyophilized mixture of Putrescine, Spermine (Cat. No. 0958) and Spermidine. The product is used as a component of the CEPT cocktail, in combination with Emricasan (Cat. No. 7310), Chroman 1 (Cat. No. 7163) and trans-ISRIB (Cat. No. 5284) to enhance viability of human pluripotent stem cells (hPSCs) and their differentiated progeny. Polyamine Supplement x1000 (lyophilized) also improves survival of pluripotent and differentiated cells during cryopreservation, organoid formation, single-cell cloning, and genome editing.

NOTE: This reagent is provided lyophilized. To use prepare a 1000x stock solution by reconstituting vial contents in 5 mL of ddH₂O and filter sterilize before use. Add 1 mL of sterile resuspended polyamines supplement (1000x stock) per 1 L of media.

Calculators

Background References

References are publications that support the biological activity of the product.

• Chen A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 2021 PMID: 33941937