Proteome Profiler Human Cell Stress Array Kit

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cell stress-related proteins. Validated for analyte detection in cell and tissue lysates.

Troubleshooting Guide

Principle of the Array

The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Lysis Buffer
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 ^°C to 8 ^°C. Do not use past kit expiration date.

Assays for analytes represented in the Human Cell Stress Array Kit

Scientific Data Examples for Proteome Profiler Human Cell Stress Array Kit

	Determination of Relative Levels of Selected Human Cell Stress-related Proteins. HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.
	Determination of Relative Levels of Selected Human Cell Stress-related Proteins. The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.