Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
R&D Systems, part of Bio-Techne | Catalog # DYC1047-2
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
62.5-4000 pg/mL
Sample Type
Cell Lysates
Reactivity
Human
Human Total p21/CIP1/CDKN1A DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total p21 / CIP1 / CDKN1A in cell lysates. An immobilized capture antibody specific for p21 / CIP1 / CDKN1A binds both phosphorylated and unphosphorylated p21 / CIP1 / CDKN1A. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
Standard Curve
Kit Contents for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Background: p21/CIP1/CDKN1A
p21/CIP1 is a Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks progression from G1 to S phase in the cell cycle. The expression of p21, a transcriptional target of p53, increases in cells where p53 is stabilized due to exposure to genotoxic agents.
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
Additional p21/CIP1/CDKN1A Products
Product Documents for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
Product Specific Notices for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
For research use only
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