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Vinculin Antibody [Alexa Fluor™ Plus 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41274AFP647

Novus Biologicals, part of Bio-Techne
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NBP2-41274AFP647
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor Plus 647

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. < Amino Acid Sequence: VREAFQPQEPDFPPPPPDLEQLR

Specificity

Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist. Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). NBP2-41274 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Vinculin Antibody [Alexa Fluor™ Plus 647]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

Additional Vinculin Products

Product Documents for Vinculin Antibody [Alexa Fluor™ Plus 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Vinculin Antibody [Alexa Fluor™ Plus 647]



This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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