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Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43866

Novus Biologicals, part of Bio-Techne
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NBP3-43866

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13403

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P45379, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free (NBP3-24557) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free

Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Troponin T Type 2 (cardiac) Antibody [NBP3-43866]

Immunocytochemistry/ Immunofluorescence: Troponin T Type 2 (cardiac) Antibody [NBP3-43866]

Staining in RH-30 cell line with Anti-Troponin T Type 2 (cardiac) monoclonal antibody) .
Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP3-43866]

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP3-43866]

Analysis in human heart muscle and skeletal muscle tissues using NBP3-43866 antibody. Corresponding Troponin T Type 2 (cardiac) RNA-seq data are presented for the same tissues.
Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP3-43866]

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP3-43866]

Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Applications for Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin T Type 2 (cardiac)

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Alternate Names

cardiac, CMD1D, CMPD2, dilated 1D (autosomal dominant), hypertrophic 2, MGC3889, RCM3, TnTc, troponin T type 2 (cardiac), troponin T, cardiac muscle

Gene Symbol

TNNT2

Additional Troponin T Type 2 (cardiac) Products

Product Documents for Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Troponin T Type 2 (cardiac) Antibody (CL13403) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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