TLR5 Antibody (19D759.2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24787
![Immunocytochemistry/ Immunofluorescence: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Immunocytochemistry-Immunofluorescence-NBP2-24787-img0012.jpg "Immunocytochemistry/ Immunofluorescence: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
![Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Immunohistochemistry-Paraffin-NBP2-24787-img0005.jpg "Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Canine
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Cytometric Bead Assay Standard, Flow Cytometry Control, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 19D759.2
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for TLR5 Antibody (19D759.2) - BSA Free
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification]![Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Flow-Cytometry-NBP2-24787-img0011.jpg "Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5 , and FITC-conjugated CD83 (right).![Western Blot: TLR5 Antibody (19D759.2)BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Western-Blot-NBP2-24787-img0006.jpg "Western Blot: TLR5 Antibody (19D759.2)BSA Free [NBP2-24787]")
Western Blot: TLR5 Antibody (19D759.2)BSA Free [NBP2-24787]
Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.![Immunohistochemistry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Immunohistochemistry-NBP2-24787-img0013.jpg "Immunohistochemistry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
![Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-BSA-Free-Flow-Cytometry-NBP2-24787-img0014.jpg "Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
![Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Immunohistochemistry-Paraffin-NBP2-24787-img0004.jpg "Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
![Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Immunohistochemistry-Paraffin-NBP2-24787-img0002.jpg "Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
![Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]](https://resources.bio-techne.com/images/products/TLR5-Antibody-19D759-2-Flow-Cytometry-NBP2-24787-img0003.jpg "Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]")
Applications for TLR5 Antibody (19D759.2) - BSA Free
Application
Recommended Usage
Dot Blot
reported in scientific literature (PMID 27248820)
Flow (Cell Surface)
reported in scientific literature (PMID 24412598)
Flow (Intracellular)
0.5-1 ug/10^6 cells. Use reported in scientific literature (PMID 24412598)
Flow Cytometry
0.5-1 ug/10^6 cells
Immunocytochemistry/ Immunofluorescence
5 ug/ml. Use reported by customer review
Immunohistochemistry
1:10-1:500. Use reported in scientific literature (PMID 27392931)
Immunohistochemistry-Frozen
10 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reviewed Applications
Read 1 review rated 4 using NBP2-24787 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TLR5
Long Name
Toll-like Receptor 5
Alternate Names
TIL-3
Gene Symbol
TLR5
Additional TLR5 Products
Product Documents for TLR5 Antibody (19D759.2) - BSA Free
Product Specific Notices for TLR5 Antibody (19D759.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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