TLR5 Antibody (19D759.2) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24783
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Canine
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Cited:
Immunohistochemistry-Paraffin, Western Blot, IF/IHC
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 19D759.2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for TLR5 Antibody (19D759.2) [PE]
Flow Cytometry: TLR5 Antibody (19D759.2) [PE] [NBP2-24783] - Intracellular flow analysis of TLR5 in human PBMCs using 1 ug/10^6 cells of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR5 antibody.
Flow Cytometry: TLR5 Antibody (19D759.2) [PE] [NBP2-24783] - Analyis of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5 , and FITC-conjugated CD83 (right).
Applications for TLR5 Antibody (19D759.2) [PE]
Application
Recommended Usage
Flow Cytometry
1ul/1 million cells
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-24787. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: TLR5
Long Name
Toll-like Receptor 5
Alternate Names
TIL-3
Gene Symbol
TLR5
Additional TLR5 Products
Product Documents for TLR5 Antibody (19D759.2) [PE]
Product Specific Notices for TLR5 Antibody (19D759.2) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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