Thrombopoietin R/Tpo R Antibody (JE58-00)

Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039]

Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039]

Western blot analysis on different lysates with NBP3-33039 at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33039 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.