Themis Antibody (719945) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # FAB6816MFV500
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 719945
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant mouse Themis
Lys122-Lys237
Accession # Q8BGW0
Lys122-Lys237
Accession # Q8BGW0
Specificity
Detects mouse Themis in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2‑282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2‑282) is observed under reducing conditions.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Themis Antibody (719945) [mFluor Violet 500 SE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Themis
THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.
Long Name
Thymocyte-expressed Molecule Involved in Selection
Alternate Names
C6orf190, C6orf207, Gasp, SPOT, TSEPA
Gene Symbol
THEMIS
Additional Themis Products
Product Documents for Themis Antibody (719945) [mFluor Violet 500 SE]
Product Specific Notices for Themis Antibody (719945) [mFluor Violet 500 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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