Skp1 Antibody (10G5X5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16734
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10G5X5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 64-163 of human SKP1 (P63208). HHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Skp1 Antibody (10G5X5) (NBP3-16734) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Skp1 Antibody (10G5X5)
![Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734]](https://resources.bio-techne.com/images/products/Skp1-Antibody-10G5X5-Western-Blot-NBP3-16734-img0002.jpg "Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734]")
Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734]
Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734] -
Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734] - Confocal imaging of NIH/3T3 cells using Skp1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for Skp1 Antibody (10G5X5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SKP1
Long Name
S-phase Kinase Associated Protein 1
Alternate Names
OCP2, p19a, p19Skp1, SKP1A
Gene Symbol
SKP1
Additional SKP1 Products
Product Documents for Skp1 Antibody (10G5X5)
Product Specific Notices for Skp1 Antibody (10G5X5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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