Seladin 1 Antibody (HL3253)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48781
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3253
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human Seladin 1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Seladin 1 Antibody (HL3253) (NBP3-48781) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Seladin 1 Antibody (HL3253)
Western Blot: Seladin 1 Antibody (HL3253) [NBP3-48781] -
Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Seladin 1 Antibody (HL3253) [NBP3-48781] -
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/ Immunofluorescence: Seladin 1 Antibody (HL3253) [NBP3-48781] -
Seladin 1 antibody [HL3253] detects Seladin 1 protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: Seladin 1 stained by Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Applications for Seladin 1 Antibody (HL3253)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Seladin 1
Alternate Names
24-dehydrocholesterol reductaseNbla03646, DCE, desmosterol-to-cholesterol enzyme, Diminuto/dwarf1 homolog, EC 1.3.1.72, KIAA0018SELADIN1, seladin 1,3-beta-hydroxysterol delta-24-reductase, seladin-1, selective AD indicator 1,3 beta-hydroxysterol delta 24-reductase
Gene Symbol
DHCR24
Additional Seladin 1 Products
Product Documents for Seladin 1 Antibody (HL3253)
Product Specific Notices for Seladin 1 Antibody (HL3253)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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