RXR alpha/NR2B1 Antibody (6Y10M1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15667
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6Y10M1 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RXR alpha/NR2B1 (P19793). MDTKHFLPLDFSTQVNSSLTSPTGRGSMAAPSLHPSLGPGIGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPTTPTLGFSTGSPQLSSPMNP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RXR alpha/NR2B1 Antibody (6Y10M1) (NBP3-15667) is a recombinant monoclonal antibody validated for use in WB, ELISA, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RXR alpha/NR2B1 Antibody (6Y10M1)
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]](https://resources.bio-techne.com/images/products/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0003.jpg "Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]")
Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]
Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]](https://resources.bio-techne.com/images/products/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0002.jpg "Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]")
Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]
Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] -
Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Confocal imaging of HeLa cells using RXR alpha/NR2B1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Applications for RXR alpha/NR2B1 Antibody (6Y10M1)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5μg antibody for 10μg-15μg of Chromatin
Chromatin Immunoprecipitation Sequencing
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RXR alpha/NR2B1
Long Name
Retinoid X Receptor, alpha
Alternate Names
NR2B1, RXRA
Gene Symbol
RXRA
Additional RXR alpha/NR2B1 Products
Product Documents for RXR alpha/NR2B1 Antibody (6Y10M1)
Product Specific Notices for RXR alpha/NR2B1 Antibody (6Y10M1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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