RPB2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87786
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (99%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RPB2 Antibody - BSA Free (NBP1-87786) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RPB2 Antibody - BSA Free
![Western Blot: RPB2 Antibody [NBP1-87786]](https://resources.bio-techne.com/images/products/RPB2-Antibody-Western-Blot-NBP1-87786-img0010.jpg "Western Blot: RPB2 Antibody [NBP1-87786]")
Western Blot: RPB2 Antibody [NBP1-87786]
Western Blot: RPB2 Antibody [NBP1-87786] - Analysis in human cell line K562.![Immunocytochemistry/ Immunofluorescence: RPB2 Antibody [NBP1-87786]](https://resources.bio-techne.com/images/products/RPB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87786-img0004.jpg "Immunocytochemistry/ Immunofluorescence: RPB2 Antibody [NBP1-87786]")
Immunocytochemistry/ Immunofluorescence: RPB2 Antibody [NBP1-87786]
Immunocytochemistry/Immunofluorescence: RPB2 Antibody [NBP1-87786] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.![RPB2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RPB2 Antibody - BSA Free [NBP1-87786]](https://resources.bio-techne.com/images/products/nbp1-87786_rabbit-polyclonal-rpb2-antibody-25620259103184.jpg "Chromatin Immunoprecipitation-exo-Seq: RPB2 Antibody - BSA Free [NBP1-87786]")
Chromatin Immunoprecipitation-exo-Seq: RPB2 Antibody - BSA Free [NBP1-87786]
ChIP-Exo-Seq composite graph for Anti-POLR2B (NBP1-87786) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for RPB2 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RPB2
Alternate Names
DNA-directed RNA polymerase II subunit RPB2, EC 2.7.7, EC 2.7.7.6, hRPB140, hsRPB2, POL2RB, polymerase (RNA) II (DNA directed) polypeptide B (140kD), polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA polymerase II subunit2, RNA polymerase II second largest subunit
Gene Symbol
POLR2B
Additional RPB2 Products
Product Documents for RPB2 Antibody - BSA Free
Product Specific Notices for RPB2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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