Ribosome maturation protein SBDS Antibody (1E8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1E8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Ribosome maturation protein SBDS (1-250aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Ribosome maturation protein SBDS Antibody (1E8) - BSA Free
Western Blot: Ribosome maturation protein SBDS Antibody (1E8)BSA Free [NBP2-22594]
Western Blot: Shwachman Bodian-Diamond syndrome Antibody (1E8) [NBP2-22594] - Lane 1. 293T cell lysate, Lane 2. HeLa cell lysate.Immunocytochemistry/ Immunofluorescence: Ribosome maturation protein SBDS Antibody (1E8) - BSA Free [NBP2-22594]
Immunocytochemistry/Immunofluorescence: Shwachman Bodian-Diamond syndrome Antibody (1E8) [NBP2-22594] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SBDS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Western Blot: Ribosome maturation protein SBDS Antibody (1E8) - BSA Free [NBP2-22594] -
Genetic reconstitution of viral infection by expression of SBDS and SPATA5. (A) Infectivity of Huh7.5 cells treated with indicated CRISPR gRNAs and complemented with lentiviral expression of firefly luciferase (Fluc) or guide-resistant host factors infected with MOI 1 YFV-Venus for 24 h and analyzed by flow cytometry. (B) Lysates from Huh7.5 cells untreated or treated with indicated CRISPR gRNAs and complemented with lentiviral expression of Fluc or guide-resistant host factors were analyzed by Western blotting using anti-SBDS or anti-SPATA5 antibodies. Blot membranes were also probed with anti-actin antibodies (indicated) (n = 3 biological replicates). Statistical significance was determined by two-way ANOVA. KO, knockout; NonTarg or NT, nontargeting CRISPR guides; WT, wild type; ****, P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36809113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Ribosome maturation protein SBDS Antibody (1E8) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ribosome maturation protein SBDS
Alternate Names
FLJ10917, ribosome maturation protein SBDS, SDSCGI-97, Shwachman-Bodian-Diamond syndrome, Shwachman-Bodian-Diamond syndrome protein, SWDS
Gene Symbol
SBDS
UniProt
Additional Ribosome maturation protein SBDS Products
Product Documents for Ribosome maturation protein SBDS Antibody (1E8) - BSA Free
Product Specific Notices for Ribosome maturation protein SBDS Antibody (1E8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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